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Optimum Temperature for Lactose Hydrolysis in Batch Reactors

reactor temperature

initial enzyme loading

initial lactose concentration

0.1

desired extent oflactose hydrolysis

0.9

At the temperature 30° C, an initial lactose concentration of 0.1 mol/liter and enzyme loading of 1g/liter, 90.0% of the lactose initially present was hydrolyzed by 11.23 hours.

The curves show the time courses of substrate lactose concentration (as a fraction of initial lactose concentration), product galactose concentration (again as a fraction of the initial lactose concentration), and the active fraction of lactase enzyme in a batch reactor for the chosen values of the manipulated variables: reactor temperature (°C), initial enzyme concentration (gram/liter), and initial lactose concentration (mol/liter).

The desired level of hydrolysis may not be always feasible, depending on your choices for the manipulated variables. Compare your desired level of lactose hydrolysis with the extent of hydrolysis achieved (or the product galactose concentration, as a fraction of initial lactose concentration) at the end of the dynamic simulation period. The goal of this interactive simulation is to achieve your desired level of lactose hydrolysis with the minimum amount of initial enzyme loading and/or minimum time necessary for batch reaction.

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